Lipoxidase from Soybean (1) (QA-825, DEAE-825)

Lipoxidase from soybean was analyzed using IEC QA-825 ( a column for strong anion exchange chromatography ) and IEC DEAE-825 ( a column for weak anion exchange chromatography ). The enzyme activity of this sample was measured by using hydrogen peroxide as a substrate. Enzyme activity was found at the arrowhead position.

Sample : Lipoxidase from soybean

 Columns : Shodex IEC QA-825 (8.0mmID*75mm) : Shodex IEC DEAE-825 (8.0mmID*75mm)
 Eluent : (A); 20mM Ethanolamine-HCl buffer(pH9.0) : (A); 20mM Tris-HCl(pH8.0)
 (B); (A) + 0.5M NaCl (B); (A) + 0.5M NaCl
 Linear gradient:0min to 60min, (A) to (B)
 Flow rate : 1.0mL/min
 Detector : UV(280nm)
 Column temp. : Room temp.