AFpak AGA-894 ( an affinity column ) which has N-acetyl-glucosamine as the ligand was used for the adsorption and elution of lysozyme.
Sample : Lysozyme
Column : Shodex AFpak AGA-894 (8.0mmID*50mm)
Eluent : (A): 0.01M TRis-HCl buffer(pH7.5) + 0.01M NaCl
(B): 0.01M TRis-HCl buffer(pH7.5) + 0.5M NaCl
Step gradient: 0min to 12min, (A) to (B)
Flow rate : 0.5mL/min
Detector : UV(280nm)
Column temp. : Room temp.
