Organic acids are by-product of hydrolysis of biomass. The organic acids are the cause of fermentation inhibitor, because these have strong growth inhibitory effect for microorganism. Acetic acid and lactic acid are analyzed with maltooligosaccharides (dimmer – tetramer) and ethanol at the same time.
SUGAR SH1821 is good for analysis of mixture of saccharides and organic acids. Eluent position of organic acids can be controlled by using sulfuric acid aqueous solution.
Sample : 20µL
1. Maltotetraose
2. Maltotriose
3. Maltose
4. Glucose
5. Lactic acid
6. Glycerol, Glycerin
7. Acetic acid
8. Methanol, Methyl alcohol
9. Ethanol, Ethyl alcohol
Column : Shodex SUGAR SH1821 (8.0mmID*300mm) Eluent : 0.5mM H2SO4 aq. Flow rate : 0.33mL/min Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 75°C
