According to USP method (United States Pharmacopeia 23), mannitol should be analyzed using a column which can separate mannitol and and sorbitol with R (resolution) of equal to or greater than 2.0. USPpak MN-431 is a column specially designed for mannitol analysis, which satisfies this criterion.
Sample :
1. Mannitol
2. Sorbitol
Column : Shodex USPpak MN-431 (4.0mmID*250mm)
Eluent : H2O
Flow rate : 0.5mL/min
Detector : Shodex RI
Column temp. : 60°C
The measurement of mannitol by USP method should be done at 30 to 85 °C within 2 °C.
