Margarine (KF-801)

Vitamin A palmitate was analyzed using GPC KF-801. Wave length of 325nm at which it has maximum UV absorption was used for the detection. Usually, saponification is necessary to analyze vitamin A as the sample pretreatment. However, in this analysis, no complicated sample pretreatment procedure was necessary. What is necessary is just to dissolve the sample in the eluent(THF) and filtrate it using a 0.45 micron disposable filter. This method using KF-801 is a very simple way to analyze vitamin A.

Sample : Margarine
1. Vitamin A palmitate

 Columns : Shodex GPC KF-801 (8.0mmID*300mm) x
 Eluent : THF
 Flow rate : 1.0mL/min
 Detector : UV(325nm)
 Column temp. : 40°C