Melamine and Related Substances (VG-50 4D)

Melamine, ammeline, ammelide, and cyanuric acid were analyzed by HILIC mode simultaneously using polymer-based amino column HILICpak VG-50 4D.

Sample : 5μL
5μg/mL each
(in H₂O/CH₃CN=50/50)
1. Melamine
2. Ammeline
3. Cyanuric acid
4. Ammelide

Column       : Shodex HILICpak VG-50 4D (4.6mmI.D. x 150mm)
Eluent       : 30mM Ammonium formate/CH3CN=35/65
Flow rate    : 0.6mL/min
Detector     : Corona charged aerosol
Column temp. : 40°C