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Category: Drugs

Methylxanthine (2)

 

Theobromine, theophylline, caffeine were separated by RSpak DE-413 (4.6mmID x 150mm) and DE-213 (2.0mmID x 150mm). As DE-213 is a semi-micro type column, it can be also used for LC/MS.

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Sample :
1. Theobromine
2. Theophylline
3. Caffeine

 (Left)
 Column : Shodex RSpak DE-413(4.6mmID*150mm)
 Eluent : H2O/CH3CN=75/25
 Flow rate : 0.5mL/min
 Detector : UV(254nm)
 Column temp. : 30°C

 (Right)
 : Shodex RSpak DE-213(2.0mmID*150mm)
 : H2O/CH3CN=75/25
 : 0.1mL/min
 : 30°C

 

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