Anions in milk were analyzed using IC I-524A.
Milk contains a considerable amount of proteins and fats, and therefore should be pretreated.
100-fold dilution with pure water was made and then treated by an solid phase extraction cartridge
for reversed phase chromatography.
Sample : 100-fold diluted Milk, 50micro-L
Sample : Anion Standards, 50micro-L
1. F- 5mg/L
4. NO2– 5mg/L
5. Br– 10mg/L
6. NO3– 10mg/L
8. SO42- 10mg/L
Column : Shodex IC I-524A (4.6mmID*100mm) Eluent : 1.5mM p-Hydoxybenzoic acid + 1.7mM N,N-Diethylethanolamine + 10% CH3OH Flow rate : 1.5mL/min Detector : Shodex CD Column temp. : 40°C