Milk (I-524A)

Anions in milk were analyzed using IC I-524A.
Milk contains a considerable amount of proteins and fats, and therefore should be pretreated.
100-fold dilution with pure water was made and then treated by an solid phase extraction cartridge
for reversed phase chromatography.

Sample : 100-fold diluted Milk, 50micro-L

2. HCO₃^–

3. Cl^–

6. NO₃^–

7. HPO₄^2-

Sample : Anion Standards, 50micro-L

1. F- 5mg/L

2. HCO₃^–30mg/L

3. Cl^–5mg/L

4. NO₂^– 5mg/L

5. Br^– 10mg/L

6. NO₃^– 10mg/L

7. HPO₄^2-10mg/L

8. SO₄^2- 10mg/L

Column : Shodex IC I-524A (4.6mmID*100mm)

 Eluent : 1.5mM p-Hydoxybenzoic acid + 1.7mM N,N-Diethylethanolamine + 10% CH3OH

 Flow rate : 1.5mL/min

 Detector : Shodex CD

 Column temp. : 40°C