Monosaccharides (3) (NH2P-50 4E)

Monosaccharides were analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis).

Sample : 10micro-L
1. 20mg/mL Mannose
2. 20mg/mL Galactose
3. 1mg/mL Glucose

 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : 10mM *Tetrapropylammonium buffer(pH10.0)/CH3CN=20/80
 Flow rate : 0.6mL/min
 Detector : Shodex RI
 Column temp. : 30°C

^*How to make Tetrapropylammonium buffer:
Please add acetic acid to 10mM Tetrapropylammonium hydroxide and adjust to pH 10.