In the Guidelines on Nutrition Labelling in Japan, amino columns of 4.6mmID x 250 mm Length are adopted for the analysis of monosaccharides, disaccharides and sugar alcohols. This chromatogram shows the analysis result of typical monosaccharide and disaccharide standards using an amino column, Asahipak NH2P-50 4E. Since the column is polymer-based, it is durable compared with silica-based columns and can be washed by alkali solution.
The eluent used is the mixture of water and acetonitrile. The elution volume can be changed by changing the mixing ratio.
Sample : 0.1% each, 20micro-L
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C