Go Back Advanced Search Page

Monovalent and Divalent Cation Standards (8) (Ethylenediamine Containing Sample)



Ethylenediamine (0.1% v/v of 50 mg/mL) is added to water samples to prevent HCl production from the reaction between residual chloride and chlorous acid. Ion chromatography (cation) column, IC
YS-50 was used to separate mono- and di-valent cations in the presence of ethylenediamine. 10% ACN was added to the mobile phase to shorten ethylenediamine retention. Addition of crown ether can improve the separation of Na+ and NH4+ , but this will increase the ethylenediamine retention.


Sample : 10µL

Cation mixture std., 10µL

1. Li+ 2mg/L

2. Na+ 10mg/L

3. NH4+ 10mg/L

4. K+ 20mg/L

5. Mg2+ 10mg/L

6. Ca2+ 20mg/L

7. Ethylenediamine, EDA 50mg/L




Column : Shodex IC YS-50 (4.6mmID*125mm)

Eluent : (A); 4mM Nitric acid aq./CH3CN=90/10
(B); 4mM Nitric acid + 1.5mM 18-Crown 6-ether aq./CH3CN=90/10

Flow rate : 1.0mL/min
Detector : Non-suppressed conductivity

Column temp. : 40°C