Sample : Myosin subfragment
Column : Shodex Asahipak ES-502C 7C (7.6mmID*100mm) Eluent : (A); 5mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.1M NaCl Linear gradient: 0min to 25min, (A) to (B) Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : 10°C
Courtesy of Dr. Muno, Juntendo University
