Myosin Subfragments (ES-502C 7C)

Myosin subfragments were analyzed using Asahipak ES-502C 7C ( a column for weak cation exchange chromatography ).

Sample : Myosin subfragment

 Column : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
 Eluent : (A); 5mM Sodium phosphate buffer(pH7.0)
 (B); (A) + 0.1M NaCl
 Linear gradient: 0min to 25min, (A) to (B)
 Flow rate : 1.0mL/min
 Detector : UV(220nm)
 Column temp. : 10°C

Courtesy of Dr. Muno, Juntendo University