N-Acetylchitooligosaccharides were analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis).
Sample: 2% Mixture of N-Acetylchitooligosaccharides, 20μL
(Seikagaku Co. code:800104 Chitooligosaccharides Mixture)
1. N-Acetyl-D-glucosamine
2. di-N-Acetylchitobiose
3. tri-N-Acetylchitotriose
4. tetra-N-Acetylchitotetraose
5. penta-N-Acetylchitopentaose
6. hexa-N-Acetylchitohexaose.
Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm) Eluent : CH3CN/H2O=70/30 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 25°C
