With Asahipak GS-310 7G (a multisolvent GPC column), a nonionic surfactant coexisting with an ionic surfactant can be measured separately from the ionic surfactants. Since the column is packed with a gel which has slight amount of carboxyl group, the ionic substance is excluded first when an eluent containing no salt is used.
GS-310 7G is an old type of GF-310 HQ.
Column : Shodex Asahipak GS-310 7G (7.6mmID*500mm)
Eluent : H2O/CH3CN=60/40
Flow rate : 1.0mL/min
Detector : UV(280nm), Shodex CD
Column temp. : 30°C