Nonylphenol ethoxylates were analyzed using MSpak GF-310 4E (a multisolvent GPC column). Nonylphenol ethoxylates are eluted by their poler using gradient solvent system.
Column : Shodex MSpak GF-310 4E (4.6mmID*250mm)
Eluent : (A); CH3CN (B); H2O
0min to 26min to 33min to 48min, A/B= 26/74 to 50/50 to 90/10 to 90/10
Flow rate : 0.5mL/min
Detector : Fluorescence detector(Ex.225nm, Em.300nm)
Column temp. : 40°C