Nutrient beverage was analyzed using RSpak DE-413, a column for reversed phase chromatography.
(Sample pretreatment)
– Dilute the nutrient beverage by 50 fold with the eluent (A).
– Pass through a 0.45μm filter before injecting into HPLC.
Sample : 20µL
| Quantification result | Concentration | Contents in 50mL |
|---|---|---|
| 1. Niacinamide |
16.5μg/mL
|
24.7mg
|
| 2. Vitamin B1 |
9.4μg/mL
|
14.1mg
|
| 3. Vitamin B2 |
2.9μg/mL
|
4.3mg
|
Column : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent : (A); 1/30M Potasium phosphate buffer(pH6.8)/CH3CN=98/2
(B); H2O/CH3CN=50/50
gradient: 0min to 4min, 100% (A),
4min to 15 min, 100% (A) to 30% (A),
After 15 min, 30% (A)
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 35°C
