Galacturonic acid is one of the uronic acids and is a component of bacterial polysaccharides such as pectin. This chromatogram shows an analysis of galacturonic acid oligomers using an Asahipak NH2P-50 4E column with phosphate buffer as the eluent. Oligogalactronic acids, from monomer to hexamer, are separated.
Sample : Oligogalacturonic acids (Courtesy of Dr. Yoshino, Kyoto Women’s University)
1. Galacturonic acid
2. Oligogalacturonic acid dimer
3. Oligogalacturonic acid trimer
4. Oligogalacturonic acid tetramer
5. Oligogalacturonic acid pentamer
6. Oligogalacturonic acid hexamer
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm) Eluent : 0.3M Sodium phosphate buffer(pH 4.4) Flow rate : 1.0mL/min Detector : UV(210nm) Column temp. : 40°C
