Saccharides in orange juice were analyzed using SUGAR SC1011 (a column for saccharides analysis). For the pretreatment of juice, first, the sample was filtrated 2 times using a 0.45μm disposal filter. Then, it was treated with a mini-column packed with anion ion exchanger. And it was filtered again. In front of glucose and fructose peaks, a large peak was observed and it was considered to be oligosaccharides.
Sample: Mandarin orange juice, 5μL
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 80°C