Organic acid standards were analyzed using RSpak KC-811.
Sample :
1. α-Ketoglutaric acid
2. 2-Ketogluconic acid
3. Citric acid
4. Tartaric acid
5. Gluconic acid
6. Malic acid
7. Succinic acid
8. Lactic acid
9. Acetic acid
10. Levulinic acid
11. Pyroglutamic acid
Columns : Shodex RSpak KC-G 8B (8.0mmI.D. x 50mm) + KC-811 (8.0mmI.D. x 300mm) x 2 Eluent : 3mM HClO4 aq. Flow rate : (Eluent); 1.0mL/min, (Reagant); 0.7mL/min Detector : VIS(430nm) (Post column method) Column temp. : 40°C
