When water or 100% buffer is used as the eluent, the column life of ODS columns is usually shorten. Since the packing material of RSpak DE-413 is chemically stable, long column life and stable analysis results can be obtained even when Water or 100% buffer was used as the eluent.
Sample :
1. Glyoxylic acid, 1.78mg/mL
2. Tartaric acid, 1.95mg/mL
3. Malic acid, 2.06mg/mL
4. Lactic acid, 2µL/mL
5. Malonic acid, 1.95mg/mL
6. Acetic acid, 2µL/mL
7. Succinic acid, 2.05mg/mL
8. Levulinic acid, 1.95mg/mL
9. Propionic acid, 2µL/mL
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : 10mM H3PO4 aq.
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 50°C