Four kinds of organic acids were analyzed by RSpak DE-213 which is packed in 2mm ID semi-micro column. In 4 minutes, sharp peaks could be obtained. For the detection, RI detector with small cell volume type was used.
Sample : 1mg/mL each , 1µL
1. Glyoxylic acid
2. Tartaric acid
3. Malic acid
4. Lactic acid
Column : Shodex RSpak DE-213 (2.0mmID*150mm)
Eluent : 10mM H3PO4 aq.
Flow rate : 0.15mL/min
Detector : Shodex RI (small cell volume)
Column temp. : 50°C
