The main organic acids and Vitamin C contained in fruit juice are separated using RSpak KC-811 with 1mM perchloric acid as the eluent.
Sample : Fruit juice
1. Cl–
2. Oxalic acid
3. Maleic acid
4. Citric acid
5. Malic acid
6. Vitamin C
7. Succinic acid
8. Formic acid
9. Acetic acid
Columns : Shodex RSpak KC-G 8B (8.0mmI.D. x 50mm) + KC-811 (8.0mmI.D. x 300mm) x 2 Eluent : 1mM HClO4 aq. Flow rate : (Eluent); 1.0mL/min, (Reagent); 0.4mL/min Detector : VIS(430nm) (Post column method) Column temp. : 40°C
