Phenols (2) (DE-413)

Phenols were analyzed using RSpak DE-413, a column for reversed phase chromatography.

Sample : 5μL
(Fluorescence) 1μg/mL each
(UV) 100μg/mL each
1. Phenol
2. 4-tert-Butylphenol
3. 4-Octylphenol
4. Nonylphenol

Column       : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 1.0mL/min
Detector     : Fluorescence(Ex. 225nm, Em. 300nm), UV(220nm)
Column temp. : 40°C