Plasminogen was analyzed using AFpak ALS-894 (an affinity column).
Sample : Plasminogen (from bovine plasma, Sigma)
Column : Shodex AFpak ALS-894 (8.0mmID*50mm)
Eluent : (A); 0.1M Sodium acetate(pH7.4)
(B); 0.1M Sodium acetate + 0.2M 6-aminohexanoic acid(pH4.0)
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
