Poly-Lysine

Basic polypeptide Poly-Lysine was analyzed using PROTEIN KW-803 (a column for GFC separation).

Sample: 100μL
Poly-L-lysine hydrobromide (Sigma-Aldrich) 0.1%

Column       : Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : 30°C