Aqueous SEC column, OHpak
SB-806 HQ
was used for the analysis of polydenylic acid.
Sample : 20µL
1. Polyadenylic acid
poly[A]n 0.1% (Wako Pure Chemical Industries, Ltd.
code:101011 POLY-ADENYLIC ACID)
Column : Shodex OHpak SB-806 HQ (8.0mmID*300mm) Eluent : 10mM Sodium phosphate buffer(pH4.6) Flow rate : 1.0mL/min Detector : UV(260nm) Column temp. : 30°C
