Polyoxyethylene cetyl ether(Brij®-56) which is a nonionic surfactant was analyzed using Asahipak GF-310 HQ ( a multisolvent GPC column ). The component of which polyethylene chain is longer elutes faster.
Sample : 1%, 20µL
Brij®-56 (Polyoxyethylene cetyl ether)
Column : Shodex Asahipak GF-310 HQ (7.6mmID*300mm)
Eluent : CH3CN/H2O=60/40
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : 40°C