Polystyrene standards were analyzed using GPC HK-402, an organic SEC (GPC) column for ultra-rapid analysis. The RI chromatograms showed that n-propylbenzene and benzene peaks overlapped with eluent-related negative peaks.
RI
Sample : 0.5 % each, 5 µL
1. Polystyrene (Mp: 21,800)
2. Polystyrene (Mp: 9,860)
3. Polystyrene (Mp: 6,940)
4. Polystyrene (Mp: 3,180)
5. Polystyrene (Mp: 1,270)
6. Polystyrene (Mp: 580)
7. n-Propylbenzene (MW: 120)
8. Benzene (MW: 78)
UV
Sample : 0.1 % each, 5 µL
1. Polystyrene (Mp: 21,800)
2. Polystyrene (Mp: 9,860)
3. Polystyrene (Mp: 6,940)
4. Polystyrene (Mp: 3,180)
5. Polystyrene (Mp: 1,270)
6. Polystyrene (Mp: 580)
7. n-Propylbenzene (MW: 120)
8. Benzene (MW: 78)
Columns : Shodex GPC HK-402 (4.6 mm I.D. x 150 mm) x 2 Eluent : THF Flow rate : 1.0 mL/min Detector : RI (small cell volume), UV (254 nm) (small cell volume) Column temp. : 25 °C
