Preservatives were analyzed using RSpak DS-613 (a column for reversed phase chromatography).
Sample :
1. Saccharin sodium
2. p-Hydroxybenzoic acid
3. Sorbic acid
4. Benzoic acid
5. Methyl paraben,
Methyl p-hydroxybenzoate
6. Ethyl paraben,
Ethyl p-hydroxybenzoate
7. Dehydroacetic acid
8. Propyl paraben,
Propyl p-hydroxybenzoate
Column : Shodex RSpak DS-613 (6.0mmID*150mm)
Eluent : (0.05M KH2PO4 + 0.1% H3PO4) aq./CH3CN=60/40
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : 22°C
