Food additives, which have wide polarity range, can be analyzed using RSpak DE-413 (a column for reversed phase chromatography) by simple isocratic mode in a short time.
Sample :
1. Saccharin sodium
2. p-Hydroxybenzoic acid
3. Sorbic acid
4. Benzoic acid
5. Methyl paraben, Methyl p-hydroxybenzoate
6. Ethyl paraben, Ethyl p-hydroxybenzoate
7. Dehydroacetic acid
8. Propyl paraben, Propyl p-hydroxybenzoate
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : (0.05M KH2PO4 + 0.1% H3PO4) aq./CH3CN=35/65
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : 40°C
