Preservatives (DS-413)

Polymer-based reversed phase column, RSpak DS-413, was used to analyze food additives including an artificial sugar, saccharin sodium, and several
preservatives.

Sample : 10micro-g/mL each, 20micro-L

1. Saccharin sodium

2. p-Hydroxybenzoic acid

3. Sorbic acid

4. Benzoic acid

5. Methyl paraben, Methyl p-hydroxybenzoate

6. Ethyl paraben, Ethyl p-hydroxybenzoate

7. Dehydroacetic acid

8. Propyl paraben, Propyl p-hydroxybenzoate

 Column : Shodex RSpak DS-413 (4.6mmID*150mm)
 Eluent : (0.05M KH2PO4 + 0.1% H3PO4) aq./CH3CN=60/40
 Flow rate : 1.0mL/min
 Detector : UV(210nm)
 Column temp. : 50deg-C