The type of preservatives allowed using in cosmetics and their limits are regulated in Japan (law released in 2000): the maximum allowance of phenoxyethanols and the sum of paraoxybenzoate esters (parabens) and their salts are both set as 1 g/ 100 g of cosmetics. Polymer-based reversed-phase column, Asahipak ODP-50 4D, was used for simultaneous analysis of 2-phenoxyethanol and 6 types of parabens. The result shows good separation of the seven components.
Sample : 10μg/mL each, 20μL
1. 2-Phenoxyethanol
2. Methyl paraben, Methyl p-hydroxybenzoate
3. Ethyl paraben, Ethyl p-hydroxybenzoate
4. Isopropyl paraben, Isopropyl p-hydroxybenzoate
5. Propyl paraben, Propyl p-hydroxybenzoate
6. Isobutyl paraben, Isobutyl p-hydroxybenzoate
7. Butyl paraben, Butyl p-hydroxybenzoate
Column : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm) Eluent : H2O/CH3CN=65/35 Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : 40°C
