The principle of affinity chromatography is as follows:
1) Inject a sample into an initially equilibrated
2) Only the substances with affinity for
the ligand are retained in the column.
3) Other substances with no affinity for
the ligand are eluted from the column.
4) The substances retained in the column
can be eluted from the column by
changing pH or salt or organic solvent concentration
of the eluent.
Affinity chromatography is widely used as
a means of separation and
purification with specific properties.