In this analysis, the sample was separated into four peaks including the transferrin peak; the largest one is serum albumin.
Sample : Human serum
4. Serum albumin
Column : Shodex IEC QA-825 (8.0mmID*75mm) Eluent : (A); 20mM Tris-HCl buffer(pH8.6) (B); (A) + 0.5M NaCl Linear gradient: 0min to 60min, 100% (A) to 50% (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.