Proteins in Human Serum (3) (KW-803)

Proteins in human serum were analyzed using PROTEIN KW-803 (a column for Aqueous SEC (GFC)).

 

Sample: Human serum, 20μL.

Sample : Protein std. 0.1% each
1. Fibrinogen 50μL
2. α2 Macroglobulin 50μL
3. IgG 50μL
4. Transferrin 50μL
5. Plasminogen 50μL
6. Albumin 100μL
7. Antitrypsin 100μL
8. Hemoglobin 100μL.

 

Columns      : Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm)
Eluent       : 50mM Phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.