Purine Bases and Pyrimidine Bases (3) (DE-613)

Six components were completely separated using RSpak DE-613 (a column for reversed phase chromatography). Analysis time can be shortened by adding acetonitrile in the eluent.

Sample :
1. Cytosine
2. Uracil
3. Hypoxanthine
4. Guanine
5. Thymine
6. Adenine

 Column : Shodex RSpak DE-613 (6.0mmID*150mm)
 Eluent : 0.05M KH2PO4 aq.
 Flow rate : 1.0mL/min
 Detector : UV(254nm)
 Column temp. : 25°C