Purine Bases, Pyrimidine Bases and Nucleosides (2) (NN-814)

Six components were separated using RSpak NN-814 with pH3.0 phosphate buffer as the eluent. Since cation exchange mode is contributing to the separation, the elution order is different from that of the separation with RSpak DE-613.

Sample :
1. Uridine
2. Uracil
3. Guanosine
4. Cytidine
5. Adenosine
6. Cytosine

 Column : Shodex RSpak NN-814 (8.0mmID*250mm)
 Eluent : 100mM NaH2PO4(pH3.0 adjusted by H3PO4)
 Flow rate : 1.0mL/min
 Detector : UV(260nm)
 Column temp. : 25°C