Pyridylamino sugar chains were separated using Asahipak
NH2P-50 4E
Refractive Index detector (RI) is generally used for sugar analyses; however
pryidylamino sugars can be detected by more sensitive fluorescence detectors.
Also the analysis can be carried under gradient elution.
Sample : : 0.125pmol/µL, 5µL
1. PA-Sugar chain 012
2. PA-Sugar chain 013
3. PA-Sugar chain 014
4. PA-Sugar chain 001
5. PA-Sugar chain 015
6. PA-Sugar chain 002
7. PA-Sugar chain 010
8. PA-Sugar chain 004
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : (A); 200mM Acetic acid-Triethylamine(pH7.3)/CH3CN=35/65
(B); 200mM Acetic acid-Triethylamine(pH7.3)/CH3CN=50/50
Flow rate : 0.5mL/min
Detector : Fluorescence(Ex: 310nm , Em: 380nm)
Column temp. : 40° C
