Comparison of protein recovery between DEAE3N-4T and DEAE-825. Both show high
recovery rate because of very low adsorption level of protein.
| Protein | IEC DEAE3N-4T Recovery(%) | IEC DEAE-825 Recovery(%) |
|---|---|---|
| Ovalbumin | 94 | 95 |
| Trypsin inhibitor | 94 | 90 |
| BSA | 95 | 92 |
| α Lactoalbumin | 99 | 98 |
| ß Lactoglobulin | 97 | 94 |
Column : Shodex IEC DEAE3N-4T (4.6mmID x 35 mm)
Shodex IEC DEAE-825 8.0mmID x 75mm)
Eluent : 20mM Tris-HCl buffer + 0.5M NaCl (pH8.0)
Flow rate : (DEAE3N-4T) 1.5mL/min
(DEAE-825) 1.0mL/min
Detector : UV (280nm)
Column temp. : 30°C
