Both PROTEIN KW-803 and Company A column (a column for aqueous SEC (GFC)) show high mass recoveries of ribonuclease A and thyroglobulin. Such high recoveries are attributed to minimum adsorption properties. Furthermore, the calibration curve obtained by sample loading and peak areas shows good linearity in a wide range between 1μg to 100μg.
Columns : Shodex PROTEIN KW-803 (8.0mmI.D. x 300mm), Company A column Eluent : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : Room temp.