Non-porous columns cannot be loaded with much sample compared with porous type
columns. Please take care of sample amount. In the case
of preparation, porous type is recommended.
Max. amount of
sample load (per column):
DEAE3N-4T: 10 µg
DEAE-825: 250 µg
HETP=column length / theoretical plate number; the
smaller HETP
means narrower peak and higher separation performance.
Column : Shodex IEC DEAE3N-4T (4.6mmID x 35 mm)
Eluent : (A); 25mM Piperazine-HCl buffer (pH6.0)
(B); (A) + 0.5M NaCl (pH6.0)
Linear gradient; (A)?(B),10min
Flow rate : 1.5mL/min
Detector : UV (280nm)
Column temp. : Room temp.
Column : Shodex IEC DEAE-825 (8.0mmID x 75mm)
Eluent : (A); 20mM Piperazine-HCl buffer (pH6.0)
(B); (A) + 0.5M NaCl (pH6.0)
Linear gradient; (A)?(B),60min
Flow rate : 1.0mL/min
Detector : UV (280nm)
Column temp. : Room temp.
