Riboflavin (Vitamin B 2 )

For the analysis of vitamin B₂, reversed phase columns are usually used with fluorescence detectors. This shows the analysis of vitamin B₂ using Asahipak GS-320 7E (a multimode column).
GS-320 7E is an old type of GS-320 HQ.

Sample :
1. Flavin mononucleotide
2. Flavin adenine dinucleotide
3. 7alpha-Hydroxyriboflavin
4. Riboflavin (Vitamin B₂)
5. Lumiflavin (LF)

 Column : Shodex Asahipak GS-320 7E (7.6mmID*250mm)
 Eluent : 1M Propionic acid buffer(pH4.4)
 Flow rate : 1.5mL/min
 Detector : Fluorescence (Ex. 250nm, Em. 520nm)
 Column temp. : 55°C