Saccharide analysis in ionic liquid (DC-613)

Mono- and di-saccharides in ionic liquid were analyzed by
RSpak DC-613.
Saccharides were separated under ligand-exchange and HILIC separation modes.
The presence of one type of ionic liquids, 1-Ethyl-3-methylimidazolium chloride,
did not interfere with saccharide separation.

Sample : 10µL, 0.1% each

1. Xylose

2. Sucrose

3. Cellobiose

4. Lactose

Sample solution:

1% 1-Ethyl-3-methylimidazolium Chloride/CH₃CN=50/50

 Column : Shodex RSpak DC-613 (6.0mmID*150mm)
 Eluent : CH3CN/H2O=70/30
 Flow rate : 0.8mL/min
 Detector : Shodex RI
 Column temp. : 50°C