Saccharides and amino acids were analyzed using SUGAR SC1011, a column for an analysis of saccharides. Generally, columns used for ligand exchange chromatography are used with water as the eluent, but adding cations as the counter ion enables the columns to separate samples under ion exchange modes well. CaSO4 aqueous solution was used as an eluent for SUGAR SC1011 with the counter ion as Ca2+, allowing ligand exchange and ion exchange to occur. Ligand exchange occurred between the counter ions of the packing material and the hydroxy groups of saccharides. Ion exchange occurred between the sulfo groups and the amino groups of the amino acids. For sensitive measurement, attention should be paid to the positions of the vacant peak due to the cations in the eluent.
Sample : 0.2% each, 10µL
4. Glutamic acid
5. Aspartic acid
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : 10mM CaSO4 aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 80°C