Saccharides in Wood (2) (SP0810)

Two sugar analysis columns, SUGAR SP0810, were used to analyze saccharides in wood. Corona charged aerosol detector (CAD) was used as a detector. Compared to Saccharides in Wood (1), the separation of arabinose and mannose was improved by adding CH₃CN to the mobile phase.
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Sample: 10μL
1. Cellobiose 1.0%
2. Glucose 1.5%
3. Xylose 0.5%
4. Galactose 0.5%
5. Arabinose 0.5%
6. Mannose 1.0%

Column       : Shodex SUGAR SP0810 (8.0mmI.D. x 300mm) x 2
Eluent       : H2O/CH3CN=87/13
Flow rate    : 0.6mL/min
Detector     : Corona charged aerosol
Column temp. : 85°C