Saccharides and sugar alcohols were separated using SUGAR SH1011 (a column for saccharides analysis). In acidic eluent such as sulfuric acid solution, dissociation of organic acids is not so much and they are reteined in the column by reversed phase mode because of the increase of hydrophobicity. However, since organic acids are partly dissociated, minus charge of packing material and plus charge of organic acids repel each other and, therefore, organic acid whose dissociation is lager elutes faster. This mechanism is called as “ion exclusion mode”. Saccharides are eluted by GFC mode.
4. Succinic acid
5. Lactic acid
7. Acetic acid
Column : Shodex SUGAR SH1011 (8.0mmI.D. x 300mm) Eluent : 5mM H2SO4 aq. Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 60°C