(Preparation of eluent)
(1) Put 0.415g of reagent grade phthalic acid and 0.278g of reagent grade tris(hydroxymethyl)aminomethane into a 1liter messflask.
(2) Dilute with purified water to make up a 1 liter solution.
(3) Dissolve using an ultrasonic vibrator.
(4) Pass through a 0.2microm membrane filter before use.
Sample : Sea water (Water)
Column : Shodex IC I-524A (4.6mmID*100mm) Eluent : 2.5mM Phthalic acid(pH4.0 adjusted with Tris(hydroxymethyl)aminomethane) Flow rate : 1.2mL/min Detector : Shodex CD Column temp. : 40°C