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SEC Analysis of Low Molecular Weight Heparin (1) (Dalteparin Sodium) (KW-803 + KW-802.5)

Low molecular weight heparin (LMWH) obtained by decomposing unfractionated heparin by chemical or enzymatic treatment is known as a blood coagulation inhibitor. In this application, dalteparin sodium which is one of LWMH was analyzed using PROTEIN KW-803 and KW-802.5 which are aqueous SEC (GFC) columns, connected in series.

Sample : 20µL
Dalteparin sodium 5.0mg/mL
(in eluent)


Columns   : Shodex PROTEIN KW-G 6B (6.0mmI.D. x 50mm) 
            + KW-803 + KW-802.5 (8.0mmI.D. x 300mm each)
Eluent    : 0.1M CH3COONH4 + 0.02% NaN3 aq.
Flow rate : 0.5mL/min 
Detector  : Shodex RI
Column temp. : 30°C