Low molecular weight heparin (LMWH) obtained by decomposing unfractionated heparin by chemical or enzymatic treatment is known as a blood coagulation inhibitor. In this application, dalteparin sodium which is one of LWMH was analyzed using PROTEIN KW-803 and KW-802.5 which are aqueous SEC (GFC) columns, connected in series.
Sample : 20µL
Dalteparin sodium 5.0mg/mL
Columns : Shodex PROTEIN KW-G 6B (6.0mmI.D. x 50mm) + KW-803 + KW-802.5 (8.0mmI.D. x 300mm each) Eluent : 0.1M CH3COONH4 + 0.02% NaN3 aq. Flow rate : 0.5mL/min Detector : Shodex RI Column temp. : 30°C