SEC Analysis of Low Molecular Weight Heparin (2) (Parnaparin Sodium) (KW-803 + KW-802.5)

Low molecular weight heparin (LMWH) obtained by decomposing unfractionated heparin by chemical or enzymatic treatment is known as a blood coagulation inhibitor. In this application, parnaparin sodium which is one of LWMH was analyzed using PROTEIN KW-803 and KW-802.5 which are aqueous SEC (GFC) columns, connected in series.

Sample : 50µL
Parnaparin sodium 10mg/mL

Columns      : Shodex PROTEIN KW-803 + KW-802.5 (8.0mmI.D. x 300mm each)
Eluent       : 0.2M solution of Na2SO4 aq. (pH5.0 adjusted with 0.05M H2SO4 aq.)
Flow rate    : 0.5mL/min
Detector     : UV(234nm), Shodex RI
Column temp. : 40°C