SEC Analysis of Antibody Drug Conjugate (LW-803)

The antibody Drug Conjugate (ADC) is a monoclonal antibody (mAb) attached to a small molecule drug via a labile chemical linker or multiple linkers. The ADCs can differentiate and target cancerous tissues from normal, healthy tissues. Although synthetically challenging, these biotherapeutic drugs are highly potent yet very efficient as opposed to traditional chemotherapies and radiotherapies. Hence, they are attracting attention as the next-generation drug.

Recently, an ADC formulation was analyzed using the aqueous SEC (GFC) column, PROTEIN LW-803. Besides monomers, a peak that appeared to be dimers was confirmed. The LW-803 is applicable for complex ADCs impurity analysis.

Sample : 20 µL
Antibody-drug conjugate
formulation 5 mg/mL
(in H₂O)
1. Dimer
2. Monomer

Column       : Shodex PROTEIN LW-803 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl/CH3CN=90/10
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C